Sixth week of Microbiology class :)

October 29, 2017 Ai 0 Comments

Hello everyone!
For this week, our lecturer makes a revision class before our test on Friday, hence we covered the topic on Introduction to Prokaryotes. What I have got from this class is we could know that the prokaryotes is defined as a group of microbe that lacks in nucleus and membrane-bound organelles. To understand the morphology of prokaryotes, we can identify it by viewing at the shape whether it is a cocci, bacilli, vibrio or spiral. For a prokaryote that is pleomorphic, it is because a certain properties in the cell wall makes it could change it shape easily compare to other organism. We also learned about the axial filaments, fimbriae, flagella, glycolcalyx and pili that is on the cell structures and how does it works in the cell.
Arrangements of Bacilli
We also have Test I on Microbiology I subject. It covers from the Introduction to Microbiology, Microscopy, Classification of Organisms and Introduction to Prokaryotes. After these topics that I have learned, I feel that I can answer the questions in the test, but still there are a lot of things I need to study and understand. 

 That's all for this week. See you next time!

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Fifth week of Microbiology class :)

October 22, 2017 Ai 0 Comments

Hello everyone!
We're now in the fifth week of semester! Yay~! But, we're not done yet. There's a long road ahead that I haven't yet finish my journey.

 Last week, Dr. Wan asked us to upload a mindmap on Introduction to Prokaryotes in the Putrablast. So, for this week class, each of the group must write one of the points regarding the topic on the whiteboard in front of the class. My group got to write about the Flagella and how does it works on the cell structure.

 Then, by group, we have to pick a microbe and make a search on the information regarding the microbe that we already chose. We have to search by the history of the microbe, the morphology, nomenclature, cell mechanism and molecular method for the microbe.

 We also have to present about the microbes that we have chosen before. All of the 9 groups will share about what microbe that we have chose and present it for 3 minutes per group. The other group are presenting about Propionibacterium acnes, Magnetotactic bacteria, Mycobacterium tuberculosis, Alcanovirax borkumensis, Halobacterium salinarum, and Pseudomonas aeruginosa. 

 My group are presenting about a microbe called Serratia marcescens or S. marcescens. What I have found out about this microbe is that it have been classified as a non-pathogenic bacteria at first, but it becomes a pathogenic after it contributes to more death. For more information about the bacteria, click on the link given: Serratia marcescens 
Serratia marcescens in agar plate

From this class, I have gained knowledge about another bacteria that have a very unique characteristic that I never heard about it. So, this week gives me a new view on microbes.

 That's all for this week. See you next week!

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Fourth week of Microbiology class :)

October 08, 2017 Ai 0 Comments

Hello everyone!
For this week, we have learned a new topic about the Classification of Organism. We are divided into groups and each person in the group would search about sub-topic in the chapter such as the taxonomy, nomenclature, phylogenetic, methods and classification. Each of us will tell our findings to the others and vice versa.

In this chapter, I found that the methods of classifying and identifying microorganisms gives a big impression to me since I also learn it in BMY3201 Basic Microbiology Techniques. There are many methods that can be used to classify and identify the microbes, for instance:
               - Differential staining
                    -> Gram staining
                    -> Acid-fast staining
                    -> Negative staining for capsules
               - Biochemical tests
                    -> Phenol red broth
                    -> Gelatin test
                    -> Starch hydrolysis
               - Serology
                    -> Western blotting
               - Phase-typing
               - Fatty acid profiles
               - DNA Base Composition
               - DNA fingerprinting
               - Nucleic acid hybridization

 Each of the methods used have their own purpose to ensure that we could study and understand more about the microbe that we want to know. These methods are useful since it could help in making a lot of discovery on microorganisms.

 That's all for this week. See you next week!

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Third week of Microbiology class :)

October 01, 2017 Ai 0 Comments

Hello everyone!
For this week, we have started a new topic that is, Microscopy. 

This topic is about microscope and what kind of microscope is suitable to use since not all microbes can be seen under the same type of microscope, right? The microscope helps us to get the magnified image of the sample and it gets to separate the details in the image. There are two types of microscope which is the light microscope that has varieties, such as:
                          - Bright-field microscope
                          - Confocal microscope
                          - Dark-field microscope
                          - Fluorescence microscope
                          - Phase-contrast microscope

There are also electron microscope that used a beam electron to produce the magnified image. For instance:
             - Scanning electron microscope (SEM)
             - Transmission electron microscope (TEM)
             - Electron cryotomography

Before using the microscope, we have to make a preparation for the specimen by making a staining to the specimen. We can use varieties of staining procedure such as:
               - Simple staining
               - Differential staining
                    -> Gram staining
                    -> Acid-fast staining
               - Special staining
                    -> Negative staining
                    -> Endospore staining
                    -> Flagella staining

This week, we also have a quiz on this topic. The question is about the types of microscope used based on the picture given. I managed to get 5 questions right out of 6 questions. 
Quiz 1
This topic is interesting as I could learn about the usage of the microscope properly and even though it's only in a picture shown by my lecturer, I feel excited to see the image of the microbe under the microscope.

That's all for this week. See you next time!

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